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KMID : 0380620070390060625
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 6 p.625 ~ p.629
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Anti-wrinkle Activity of Low Molecular Weight Peptides derived form the collagen Isolated from Asterias amurensis
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Kwon Min-Cheol
Kim Cheol-Hee
Kim Hyo-Sung
Abdul Qadir Syed
Hwang Bo-Yong
Lee Hyeon-Yong
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Abstract
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This study was carried out to investigate the anti-wrinkle effects of peptides derived from collagens isolated from Asterias amurensis, which was collected in the East Sea. The molecular weights of the peptides were between 10-50 kDa, as determined through sephadex G-75 gel. The cytotoxicities against CCD-986sk cells and HEL-299 cell were measured using the MTT assay. The cytotoxicity of all the fractions(F1: Fraction No. 4-13, 166kDa; F2: Fraction No. 25-30, 100 kDa; F3: Fraction No. 45-55, 58kDa; F4: Fraction No. 59-63, 43kDa; F5: Fraction No. 79-90, 24kDa) was less than 25%, by the addition of 1.0mg/mL. These peptides did not show any adverse effects on human skin cells. In the presence of F1 at 1.0mg/mL, matrix metalloproteinase-1 (MMP-1) expression of UVA-induced human normal fibroblsts was reduced to 34.8%. OVerall the results seem to suggest that peptides of approximately 20kDa have superior anti-wrinkle effects.
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KEYWORD
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Asterias amurensis, low molecular weight peptide, anti-wrinkle activity
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